Effects of 2,4-Dichlorophenoxyacetic Acid on The Induction of Callus from Cotyledon and Hypocotyl Explants of Butterfly Pea (Clitoria ternatea)

Clitoria ternatea (Butterfly pea) is a tropical medicinal and fodder legume from the Fabaceae family possessing various beneficial phytochemical compounds linked to mammalian neuroprotective mechanism. Callus cell suspension cultures are excellent alternatives for harnessing secondary metabolites plants. The current study aims induce callus cotyledon hypocotyl explants of C. establishment cultures. Cotyledon two-weeks-old seedlings were subjected half-strength MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.5 mg/L 2.5 mg/L) scoring morphology assessed week 8 culture. Results revealed that treatment 0.5 2,4-D resulted in highest percentage induction (100%) both friable morphology. exhibited higher relative value 3.03 ± 0.20 compared 1.80 0.12. This thereby provides basis future studies on production valuable memory enhancing properties plant.